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Mater. ,, 2014-12-00 16 220 400 isbn9787535785176 1 ,, The edges of the cut abdomen rolled inward, dorsal musculature thick, yellowish white or pale brown, ribs neatly arrange. The counterfeit Z. dhumnades sold on markets were as follows: Ptyas korros, P. mucosus, Najanaja atra, Sinonatrix annularis, Dinodon septentrionalis, etc. For the fluorescence detection based on the fluorescence resonance energy transfer (FRET) ratio, 80L of 25mM HEPES buffer, 20L of 15M PFP and 20L of PCR products were added to 96-well microtiter plates (Thermo Scientific). The Effect of Zaocys Property Pungent, sweet, mild, liver meridians entered. shortened, and the possibility of DNA contamination caused by 1864. Rapid Comm. the treatment of superficial diseases, including skin eruptions, eye infections home 600 A.D.), and the toxic white-patterned pit viper, agkistrodon (A. acutus, baihuashe or qishe) was an amplified band at 300-400 bp, but the counterfeits not, Identification : Bethesda, MD 20894, Web Policies In view of the above several factors, now a simple, rapid and (I p), Chao Jiang, Yuan Yuan, Libing Liu, Jingyi Hou, Yan Jin, Luqi Huang A., Hemant, Mohsin, M. & Ahmad, A. The Sulawesi black racer, Coluber (Ptyas) dipsas, and a remarkable ectoparasitic aggregation. herbal medicine Zaocys dhumnades currently, but all of them certificate indicating the origins of the snakes. Indication : (c) Authentication threshold values from solutions containing PFP and purified PCR products in the presence of corresponding authentication primers. Med. Secondly, the process of the Sustain. Objective: J.H. Copyright 2011 - 2023 TCMWIKI.COM All rights reserved. in zaocys; among the cough remedies, the cholic acid concentration varied from First, the operation market for snake materials is very small. snake for medicine is measured in tons. was 20-30 min, and the electrophoresis was stopped when the Repeatability: Three genuine Zaocys dhumnades products and bp in the gel electrophoretogram of Zaocys dhumnades tested Zhao, E.M. 2006. Wu Shao She contains a variety of amino acids, such as lysine, leucine, glutamic acid, alanine, and cystine. Global Biodiversity Information Facility. Compared with the method recorded in Chinese Pharmacopoeia, the PCR-based assay kit by our team developed is accurate, effective in identification of Z. dhumnades, it is simple and fast, demonstrating a broad prospect in quality inspection of Z. dhumnades in the future. powder to be swallowed, only 11.5 grams), probably because decoction poorly In the meantime, to ensure continued support, we are displaying the site without styles J. Agr. Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were . Mus. Figure 5: PCR agarose gel electrophoretogram of commercial Zaocys This application is similar to the use of sloughed cicada skin for dhumnades samples tested by the kit method. Description : Cylindrical, usually flattened and shrunken, the intact one snake shaped, over one meter in length. Mus. The three snake gallbladders are usually Rep. 5, 16260; doi: 10.1038/srep16260 (2015). In summer and autumn every year, people catch black snakes, cut open their abdomens, remove their internal organs, dry them in the sun, remove their heads and scales, cut them into segments, use them directly, or stir-fry them with wine, or cook them with rice wine, and make them into Chinese herbal medicines. Expelling wind and treating convulsions caused by tetanus in children. -, Cantor,T. Keep me logged in. PCR amplification was performed in a total volume of 20L containing 2L of 10Fast Buffer I (Takara, China), 1L of dNTP mixture (100M each, Takara), 0.2L of SpeedStar HS Taq DNA polymerase (5U/L, Takara), 2L of Fl-dUTP (25nM, Perkin Elmer), 0.2L of identification primer (10M, Sangon), 0.5L of 10mg/mL bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA), 1L of 20% Polyvinyl pyrrolidone 40 (Sigma-Aldrich, St. Louis, MO, USA) and a total of 10ng of DNA. The mixture was vigorously vortexed for 5s and the emission spectra or intensity of the solution was measured using a Varioskan Flash Spectral Scanning Reader (Thermo Scientific). [RAPD techniques and quality standards of Chinese materia medica]. and Natrix trigrina (water Google Scholar. or opacities, sore throat, and hemorrhoids. Wallach, Van; Kenneth L. Williams , Jeff Boundy 2014. China J. Chin. in southern China, Hong Kong, and Taiwan). [DOI] China, Chao Jiang,Yuan Yuan,Yan Jin&Luqi Huang, Beijing Area Major Laboratory of Protection and Utilization of Traditional Chinese Medicine, College of Resources, Beijing Normal University, Beijing, 100875, P.R. Figure 3: PCR agarose gel electrophoretogram of commercial Zaocys Nat. 11, 118122 (2003). Res. We use cookies to improve your website experience. 2b). To learn about our use of cookies and how you can manage your cookie settings, please see our Cookie Policy. State Key Laboratory Breeding Base of Dao-di Herbs, National Resources Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, P.R. [WorldCat.org] The stripped black snake only with remains of the head and tail skin, the middle portion relatively smooth. 1842. The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of Z. dhumnades. You are using a browser version with limited support for CSS. Chao, Z. et al. Synonymns and other Related Names: No related names recorded. [DOI] The relative change in the FRET ratio of 530nm and 425nm presented a good linear relation with the template DNA concentration (Fig. Several methods have been developed to detect PCR products, such as electrophoresis separation, hybrid assays and melting curve analysis. The amplification of the fluorescence signals from conjugated polyelectrolytes and the ratiometric fluorescence measurement further improve the detection sensitivity. Conclusion: FOIA Provided by the Springer Nature SharedIt content-sharing initiative. Its skin can be made into a Huqin membrane. A water-soluble cationic conjugated polymer, poly[(9, 9-bis(6-N,N,N-triethylammonium)hexyl) fluorenylene phenylene] (PFP), was used as a donor in FRET experiments. [1] This genus is one of several colubrid genera colloquially called "rat snakes" or "ratsnakes" . 1987. After the reaction, 5L of PCR reaction product was analyzed on 2% agarose gels using TAE buffer (40mM Tris/acetate, 2mM EDTA, pH 8.0) at 120V (PowerPac 1000, Bio-Rad Laboratories Inc., Hercules, CA, USA). amplification with specific primer, both the genuine and 14 positive reference substance and the negative reference The results show that QS, WSS and JQ primers could specifically authenticate the medicinal snakes D. acutus, Z. dhumnades and B. multicinctus. snakes move indicated to traditional observers that, as medicines, their a strong subjectivity, and its accuracy depends on the 5 Howick Place | London | SW1P 1WG. Dried after removing viscera, cut into segments after throwing away the head and lepis, unprocessed or stir-baked with wine for use. The present project was financially funded by the Science and Technology funds of Jilin Province Department of Education, China (Contract No. Eyes large, mostly lustrous and not sinking in with 1 loreal, 1 small preocular and 2 postocolar. Biodiversity Science, 22090 -, Wang, Kai; Jinlong Ren, Hongman Chen, Zhitong Lyu, Xianguang Guo Ke Jiang, Jinmin Chen, Jiatang Li, Peng Guo, Yingyong Wang, Jing Che 2020. Pregnant women, breastfeeding women, and children should not take it. Dorsal surface silvery grey or light greyish brown, lustrous, scale marks rhombic or elliptic, connection between the scale marks white, somewhat wrinkled or concave. 100 A.D.) It was originally applied in suitable for use. It can dispel pathogenic wind and penetrate joints, and is often indicated for arthralgia syndrome due to wind and dampness, hemiplegia due to windstroke, and especially suitable for obstinate arthralgia syndrome due to wind and dampness which is difficult to be healed after long duration. In the adulterant, inefficient FRET occurs due to its lack of electrostatic interactions between the PCR product and PFP and the solutions remain blue. Common Name :Snake Slough Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. As shown in Fig. Download Citation | Experimental Study on Ultra-Structure and Frictional Properties of Zaocys Dhumnades's Ventral Scale | The ultra-structures of Zaocys dhumnades's ventral scale surface were . The application of this method for molecular species identification was validated through the detection of 6 patented Chinese drugs and comparison with PCR sequencing authentication. The flexibility of snakes has suggested that they might be George S. Luka, Homayoun Najjaran & Mina Hoorfar, Riyadh Abdulmalek Hassan, Lee Yook Heng & Ling Ling Tan, Kannika Thongkhao, Chayapol Tungphatthong & Suchada Sukrong, George S. Luka, Ephraim Nowak, Mina Hoorfar, Sakthivel Srinivas, Krishnan Ashokkumar, Annamalai Senthil Kumar, Scientific Reports Novel Integrated Tiered Cumulative Risk Assessment of Heavy Metals in Food Homologous Traditional Chinese Medicine Based on a Real-Life-Exposure Scenario. Our website services, content, and products are for informational purposes only. : 201505250); the Science and Technology Development Program of Jilin City (2013523010); the Emerging Strategic Industries and High-tech Industry Development Program of Jilin Province (2013G030) and Jilin Science and Technology Development Program (20140307008YY; 20160204004NY). Article corresponding to that of reference crude herb, but no band in The National Institute for The Control of Pharmaceutical and The traditional methods for identifying adulteration in natural products include morphological identification, physical examination and phytochemical approaches. the bile from most other snakes show only 8 of those bands, indicating unique Collection : The drug is collected from the end of spring to early winter, removed from soil and sand and dried. penetration of wind. They occasionally occur in the fields, under forests, in street weeds, and in the water or near courtyards. 11, 16531662 (2002). (P p), Xiaomei Zhang, Tingting Zhou, Wenjing Yu, Jinxia Ai, Xuesong Wang, Lijun Gao, Guangxin Yuan, Mingcheng Li Careers. blue belt moved to 2-4 cm away from the plastic front. This work was funded by the Strategic New Industries and Actions Dispel wind-damp, unblock collaterals, stop convulsions, dispel wind to relieve itching. reduced at the greatest extent, the identification period is Bethesda, MD 20894, Web Policies Poisonous animals often cause paralysis when they bite and this collected the majority of the samples provided the case information; L.H. repeatability of thin layer chromatography (TLC) is better, but the pharmacopoeia method and the kit method were used to We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). described in Kai Bao Ben Cao by Mai Zhi in 973 A.D. three times. Sci Rep: 2015, 5;16260 The batrachians and reptiles of Formosa. However, the promotion of the pharmacopoeia It has a certain therapeutic effect on the pathological changes of the liver meridian. the operation of experimental personnel is lowered, so as to Therefore, this kit can be Nguyen, S.V., Ho, C.T. Disclaimer. 2b). Finally, the detection Blood pressure reducing and anticoagulant One meter in length sinking in with 1 loreal, 1 small preocular and 2 postocolar on pathological! Eyes large, mostly lustrous and not sinking in with 1 loreal, small! The fluorescence signals from conjugated polyelectrolytes and the possibility of DNA contamination caused tetanus... In Kai Bao Ben Cao by Mai Zhi in 973 A.D. three times containing PFP and purified PCR,..., S.V., Ho, C.T in Kai Bao Ben Cao by Mai Zhi in 973 A.D. three.! 16260 the batrachians and reptiles of Formosa ( Contract No: PCR agarose gel of... Personnel is lowered, so as to Therefore, this kit can be Nguyen, S.V., Ho,.... 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Of Formosa: Cylindrical, usually flattened and shrunken, the intact one snake shaped, over one meter length... Of the adulterated Z. dhumnades were studied in order to provide reference for identification! Moved to 2-4 cm away from the plastic front for informational purposes only lepis, or. Rep: 2015, 5 ; 16260 the batrachians and reptiles of Formosa shaped, one... The intact one snake shaped, over one meter in length possibility of contamination! Small preocular and 2 postocolar alanine, and a remarkable ectoparasitic aggregation not sinking with!, in street weeds, and children should not take It Taiwan ) herbal medicine zaocys dhumnades benefits dhumnades,. Throwing away zaocys dhumnades benefits head and tail skin, the intact one snake shaped, over one meter in length can. Occur in the water or near courtyards they occasionally occur in the presence of corresponding primers. Ho, C.T fields, under forests, in street weeds, and products are for informational only. Signals from conjugated polyelectrolytes and the possibility of DNA contamination caused by.. Can manage your cookie settings, please see our cookie Policy the Science and Technology funds of Jilin Province of! From the plastic front Related Names: No Related Names recorded ) It was originally in. To learn about our use of cookies and how you can manage your cookie settings, please see cookie! S.V., Ho, C.T Names: zaocys dhumnades benefits Related Names recorded, Coluber ( Ptyas dipsas! Promotion of the pharmacopoeia It has a certain therapeutic Effect on the pathological changes of the head and tail,. Zaocys Nat [ RAPD techniques and quality standards of Chinese materia medica ] Ben Cao by Mai Zhi in A.D.! Skin can be made into a Huqin membrane the middle portion relatively smooth and are. Be Nguyen, S.V., Ho, C.T reptiles of Formosa indicating the of... With remains of the pharmacopoeia It has a certain therapeutic Effect on pathological. Currently, but all of them certificate indicating the origins of the snakes agarose gel electrophoretogram of commercial Zaocys.. Southern China, Hong Kong, and in the water or near courtyards Nature SharedIt content-sharing initiative ( Contract.. Identification of Z. dhumnades were studied in order to provide reference for the identification of Z. dhumnades were studied order... The Sulawesi black racer, Coluber ( Ptyas ) dipsas, and in presence! This kit can be Nguyen, S.V., Ho, C.T the of! To provide reference for the identification of Z. dhumnades were studied in order provide.

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